The Amathole Museum, a Natural and Cultural History Museum, has an international reputation, and is situated in King Williams Town, Eastern Cape Province, South Africa. It was founded in 1884.

The name of the museum has changed twice. In the spirit of transformation, the name was changed in 1999 from Kaffrarian to Amathole. The latter term refers to calves on the Mathole mountains.

Behind the doors of historic buildings, the museum houses material which is of great scientific, historical and anthropological value to researchers.